Cooper, Robin*

Owned by UKY CCS RCD
Jun 09, 2024
6 min read

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Project Title- Analysis of dopamine homeostasis at the nucleic acid level using the Drosophila Genetic Reference Panel


Overview of the project-
The purpose of this project is to identify cell-signaling pathways that influence the function of dopamine. Dopamine is a neurotransmitter that acts as a trophic factor for and regulates the excitability of neurons. We observed that among a set of 39 naturally selected populations, there is significant variation in the expression levels of dopamine-related genes. One bioinformatic approach we are using to characterize these differences is expression quantitative trait locus (eQTL) analysis of this microarray dataset. The prediction is that DNA sequence polymorphisms, in genes that are directly and indirectly related to dopamine signaling, influence the expression of dopamine-related genes.

Overview of computational methods-
Managing and analyzing the SNP and microarray datasets requires the DLX cluster. A large memory allocation is needed to generate SAS databases with these data. Those databases can then be used to generate a JMP Genomics database that is used to perform SNP-trait analysis. Each of these methods is currently available at the University of Kentucky.

Softwares

SAS 9.3-64 bit
JMP Genomics 6.0- 64 bit

Students

Titlow, Josh

Collaborators

Statement of researchers participating in this project-This project is part of a dissertation for Josh Titlow in the Biology Department at UK. The P.I. is Dr. Robin Cooper in the Biology Department. Dr. Jeremiah Smith in the Biology department is also a collaborator.

Publications

2013

  1. Bierbower, S.M., Shuranova, Z. P., Viele, K. and Cooper, R.L. (2013) Sensory systems and environmental change on behavior during social interactions. Brain and Behavior 3(1): 4–13.
  2. Becnel, J., Johnson, O., Majeed, Z.R., Tran, V., Yu, B., Roth, B.L., Cooper, R.L., Kerut, E.K., Nichols, C.D. (2013) DREADDs in Drosophila: A Pharmacogenetic Approach for Controlling Behavior, Neuronal Signaling, and Physiology in the Fly. Cell Reports 4(5):1049-59. doi: 10.1016/j.celrep.2013.08.003.
  3. Wu, W.-H. and Cooper, R.L. (2013) Physiological separation of vesicle pools in low- and high-output nerve terminals. Neuroscience Research 75:275–282. 5-Year Impact Factor: 2.376
  4. Bierbower, S.M., Nadolski, J. and Cooper, R.L. (2013) Sensory systems and environmental change on behavior during social interactions. International Journal of Zoology. Vol: 2013, Article ID 573802, 16 pages http://dx.doi.org/10.1155/2013/573802(external(external link) link)
  5. Bierbower, S.M. and Cooper, R.L. (2013) The mechanistic action of carbon dioxide on a neural circuit and NMJ communication. Journal of Experimental Zoology 319A:340–354. http://onlinelibrary.wiley.com/doi/10.1002/jez.1798/full(external(external link) link)
  6. Majeed, Z.R., Nichols, C.D., and Cooper, R.L. (2013) Pharmacogenetic approach in directing inhibition of the larval heart in Drosophila melanogaster. (In press, Journal of Applied Physiology)
  7. Majeed, Z.R., Titlow, J., Hartman, H.B. and Cooper, R.L. (2013) Proprioception and tension receptors in crab limbs: Student laboratory exercises. Journal of Visualized Experiments (JoVE). (80), e51050, doi:10.3791/51050 Professional movie and peer reviewed manuscript. http://www.jove.com/video/51050/proprioception-tension-receptors-crab-limbs-student-laboratory(external(external link) link)
  8. Titlow, J., Majeed, Z.R., Nicholls, J.G. and Cooper, R.L. (2013) Identifiable neurons in the central nervous system of a leech via electrophysiology and morphology, sensory field maps in skin and synapse formation in culture: Student laboratory exercises. Journal of Visualized Experiments (JoVE). (81), e50631, doi:10.3791/50631. Professional movie and peer reviewed manuscript. http://www.jove.com/video/50631/intracellular-recording-sensory-field-mapping-culturing-identified(external(external link) link)
  9. Titlow, J., Majeed, Z.R., Hartman, H.B., Burns, E., and Cooper, R.L. (2013) Neural Circuit Recording from an Intact Cockroach Nervous System Journal of Visualized Experiments (JoVE). (81), e50584, doi:10.3791/50584 (2013). Professional movie and peer reviewed manuscript).http://www.jove.com/video/50584/neural-circuit-recording-from-an-intact- cockroach-nervous-system
  10. Titlow, J.S., Rufer, J., King, K., and Cooper, R.L. (2013) Pharmacological analysis of dopamine modulation in the Drosophila melanogaster larval heart. Physiological Reports 1 (2), 2013, e00020, doi:10.1002/phy2.20
  11. Cooper, A.-S., Johnstone, A.F.M. and Cooper, R.L. (2013) Motor nerve terminal morphology with unloading and reloading of muscle. In Press, Journal of Crustacean Biology..
  12. deCastro, C., Titlow, J., Majeed, Z.R., and Cooper, R.L. (2013) Analysis of various physiological salines for heart rate, CNS function, and synaptic transmission at neuromuscular junctions in Drosophila melanogaster larvae (In Press, Journal of Comparative Physiology A).
  13. Majeed, Z.R., Stacy, A., and Cooper, R.L. (2013) Pharmacological identification of serotonin receptor subtypes on Drosophila larval heart. (In Press, Journal of Comparative Physiology B-).
  14. Titlow, J.S., Rice, J., Majeed, Z.R., Holsopple, E., Biecker, S., and Cooper, R.L. (2013) Modified mechanosensory assay reveals genotype-specific and anatomical differences in response to tactile stimuli: Introducing the HAT touch assay. (In Review, Journal of Neuroscience Methods).
  15. Crum, M.R., Nadolski, J., Robinson, A., Burns, E., Robinson, M.M., DeCastro, L. and Cooper, R.L. (2013) Physiological effects of acute hypercalcemia in a crayfish model: behavior, cardiac, neurophysiology, gastrointestinal. (In Manuscript).
  16. Cooper, R.L., Nadolski, J., Krall, R.M., Smith, L.A., Cooper, H.W. and Holsinger, R.C.(2013) Content dependent presentation with hands-on activity for understanding buffering related to respiration for high school and introductory college courses in biological sciences. (In Manuscript).
  17. Cooper, R.L., Krall, R.M., Nadolski, J., Smith, L.A., and Holsinger, R.C (2013) Confidence assessment and content understanding with multiple choice examinations: Biological content. (In Manuscript).
  18. Bierbower, S.M., Spence, T., Robinson, M. and Cooper, R.L. (2013) The autonomic response as in indicator of chemosensory sensitivity in multiple sensory modalities in blind and sighted crayfish. Experiments complete. (In Manuscript).
  19. VanDyke, R., Viele, K. and Cooper, R.L. (2013) Mixtures of Self-Modeling Regressions. (In Revison)
  20. Bierbower, S.M., Holmes, K. and Cooper, R.L. (2013) Effects of hypoxia on intermittent exercise in crayfish Procambarus clarkii. Experiments and Data Analysis complete. (In Manuscript).
  21. Wigginton, A.J., Bierbower, S.M., Xu, S. and Cooper, R.L. (2013) Behavioral and physiological effects of cadmium on crayfish. (In Manuscript).
  22. Anderson, H.M. and Cooper, R.L. (2013) Heart rate measurement in crayfish: Environmental changes, social interaction, and physiological response. (Revised submission). Peer reviewed laboratory exercise developed with a NSF- RET (Research Experience for Teachers) grant.
  23. Cooper, R.L. Mykles, D.L., Braxton, R., Florence, C. and Adams, K. (2013) Disuse atrophy in crayfish abdominal muscles as a result of restrictive containment. (Dr. Mykles is a collaborator at Colorado State Univ. and an expert in running SDS gels of crustacean muscle as well as probing with specific antibodies to muscle proteins. Braxton was an undergraduate. Florence and Adams were high school students working for a summer in my laboratory). (In Manuscript).
  24. Titlow, J., Turner, A.C. and Cooper, R.L. (2013) Reduced dopamine production in a Drosophila disrupts learning in larva and reduces stress tolerance in larva and adults (In manuscript).
  25. Winslow, J.L., Cooper, R.L., and Atwood, H.L. (2013) Influence of presynaptic active zone shape on the calcium response activating evoked neurotransmission. (In manuscript)
  26. Mercier, A.J. and Cooper, R.L. (2013) The differential effects of a crayfish neuropeptide on synaptic transmission of phasic and tonic motor nerve terminals (In manuscript).
  27. Bierbower, S.M. Xu, S., Wigginton, A.J. and Cooper, R.L. (2013) Behavioral and physiological effects of insecticides (pyrethrin and carbaryl) on Procambarus clarkii. (In Manuscript).

2012

  1. Holsinger, R.C., and Cooper, R.L. (2012). Effect of Environment and Modulators on Hindgut and Heart Function in Inverte¬brates: Crustaceans and Drosophila. Tested Studies for Laboratory Teaching, Volume 33 (K. McMahon, Editor). Proceedings of the 33rd Conference of the Association for Biology Laboratory Education (ABLE). http://www.ableweb.org/volumes/vol-33/v33reprint.php?ch=7(external(external link) link)
  2. Wu, W.-H. and Cooper, R.L. (2012) The regulation and packaging of synaptic vesicles as related to recruitment within glutamatergic synapses. Neuroscience 225:185–198. Impact 3.380
  3. Wu, W.-H. and Cooper, R.L. (2012) Role of serotonin in the regulation of synaptic transmission in invertebrate NMJs. Experimental Neurobiology 21(3):101-112.
  4. Chung,Y-S. Cooper, R.M., Graff, J. and Cooper, R.L. (2012) The acute and chronic effect of low temperature on survival, heart rate and neural function in crayfish (Procambarus clarkii) and prawn (Macrobrachium rosenbergii) species. Open Journal of Molecular and Integrative Physiology 2:75-86.

2011

  1. Johnstone, A.F.M., Viele, K., and Cooper, R.L. (2011) Structure/Function assessment of crayfish neuromuscular junctions. SYNAPSE 65(4):287-299. doi: 10.1002/syn.20847, Impact factor: 3.220
  2. Cooper, A.S., Leksrisawat, B., Gilberts, A.B., Mercier, A.J. and Cooper, R.L. (2011) Physiological experimentations with the crayfish hindgut. Journal of Visualized Experiments (JoVE). Jove 47: http://www.jove.com/video/2324/physiological-experimentation-with-crayfish-hindgut-student(external(external link) link) doi: 10.3791/2324.
  3. Robinson, M.M., Martin, J.M., Atwood, H.L. and Cooper, R.L. (2011) Modeling biological membranes with circuit boards and measuring conduction velocity in axons: Student laboratory exercises. Journal of Visualized Experiments (JoVE). Jove. 47: e2325 http://www.jove.com/video/2325/modeling-biological-membranes-with-circuit-boards-measuring(external(external link) link) doi: 10.3791/2325
  4. Baierlein, B., Thurow, A.L., Atwood, H.L. and Cooper, R.L. (2011) Membrane potentials, synaptic responses, neuronal circuitry, neuromodulation and muscle histology using the crayfish: Student laboratory exercises. Journal of Visualized Experiments (JoVE). Jove 47: http://www.jove.com/video/2322/membrane-potentials-synaptic-responses-neuronal-circuitry(external(external link) link) doi: 10.3791/2325.
  5. Cooper, R.M., Schapker-Finucane, H. Adami, H. and Cooper, R.L. (2011) Heart and ventilatory measures in crayfish during copulation. Open Journal of Molecular and Integrative Physiology 1(3): 36-42 http://www.scirp.org/journal/PaperInformation.aspx?paperID=8344(external(external link) link)


Grants

Center for Computational Sciences