Santamaria, Mauro Pedrine

Owned by UKY CCS RCD
Jun 09, 2024


PI: Pedrine Santamaria, Mauro

Center of Oral Health Research


Santamaria`s Laboratory

Introduction:

Lab Research Activities includes in-silica analysis from randomized clinical trials samples to better understand oral wound healing. Non-healing wounds are a health public issue worldwide. US has been spending at least 28.1 billion dollars with curative approaches to treat delayed wound healing. Biomarkers and oral microbiome may influence healing steps. Indeed, according to previous papers focusing on skin wound, microbiome profile and pro-inflammatory cytokines have a key role in wound closure. Members of this present research team has been investigating undesired wound healing and host modulation to achieve a better tissue recover. A strong focus is the determination of patient`s microbiome profile and possible interactions between biomarkers and microorganism throughout the repair stages. A key approach for it is the metagenomics and microbiome analysis combined with principal component analysis to evaluate interactions between cytokines/chemokines and oral microbiome profile during the healing process.

Projects:

  1. Understanding Mechanisms of Oral mucosa wound healing: A step toward precision therapy for vulnerable populations
  2. A New Drug Delivery System – Silk fibroin film loaded or not with insulin on palatal mucosa wound healing

Computation Methods:

Computation involves 16S rRNA gene and shotgun sequencing reads analysis (large and complex datasets) using Mothur/Qiime2 software focusing on taxonomy and OTUs. Several analyses in regards of alpha and beta diversity, Linear Discriminant Analysis, and principal components analysis will be performed either with the mentioned software or R.

Students:

PhD Candidate: Manuela Maria Viana Miguel, Added on MCC cluster, 02/17/2023  

Postdoc: Dr. Renato Correa Viana Casarin, Added on MCC cluster, 02/17/2023 

Collaborators:

Dr. Luciana Macchion Shaddox

Dr. Jin Chen, IBI, Added on MCC cluster, 05/08/2023

Luciana Moffato, Added on MCC cluster, 07/17/2023 


Software:

R

Mothur

Qiime2

PhyloToAST



Center for Computational Sciences