BIO520_S23


Instructor:  Jeramiah J Smith


Instructor:

Dr. Jeramiah J Smith


Office Address:

200 TH Morgan Building


Email:

jjsmit3@uky.edu


Office Phone:

257-0124


 



Office Hours:

Tuesday 1:00 to 2:00 PM and by appointment



Course Description:

BIOINFORMATICS

 


Prerequisites:

BIO 315 or BIO 304 or BCH 304 or BCH 401 or BCH 501 or BCH 502 or BIO 510 or consent of instructor.




Student Learning Outcomes: 


After completing this course, the student will be able to:


1.  Interact with the terminal, install and use programs.


2.  Perform analyses of large datasets (millions to billions of sequences).


3.  Write and edit simple programs/scripts (e.g. bash)





Course goals or objectives: To achieve proficiency on computational analysis of large biological datasets






Course Assignments


Class Participation                                       500 points total

3 Quizzes                                                        150 points total


              1 Pre-proposal Presentation                    100 points


1 Written Assignment (midterm)           150 points


1 Final Project                                               100 points


              Total                                                                 1000






Summary Description of Course Assignments



Class Participation

All students are expected perform the exercises covered in each in class. Because teaching someone else is the best way to learn, students that finish early are expected to assist those that have not yet completed their exercises (BUT, do not do it for them).



Pre-proposal Presentation - A 10 minute presentation outlining the basic biological question you are interested in, the specific dataset(s) you plan to analyze and the analyses you plan to perform (what you hope to learn and why). 


Notes:

              A -  if you are planning to analyze a dataset of you own that is yet to be sequenced, you must provide an alternate dataset.

              B -  You may NOT use microarray data as your primary data source, although microarrays analyses may be used to assess or complement other analyses.

                                                  

 

Written Assignment (due by 2/25/22)

 

Written assignments should be prepared in the following format:


1) a brief abstract that outlines the biological question you are interested in. (1.5-2 pages, single spaced 12-point font). You should include images, these and items listed below do not count to page limits.

 

2) list of specific datasets that you will use, including A) links to datasets on NCBI on another server (or MD5 checksums for your own sequence data), B) Molecules targeted for sequencing (DNA, RNA, mRNA, protein …), C) the type of sequencing (paired-end, single-end, etc), D) sequencing chemistry used to generate the dataset(s), E) numbers of reads/entities for each sample.


At least one concise supporting image (with title and legend) is strongly encouraged and may be embedded in text or placed at the end of the document, images need not count toward the page limit and should not be excessively large. 


* IF YOU ARE PLANNINIG TO ANALYZE A DATASET OF YOU OWN THAT IS YET TO BE SEQUENCED, YOU MUST PROVIDE AN ALTERNATE DATASET.


* You may NOT use microarray data as your primary data source, although microarrays analyses may be used to assess or complement other analyses.





Presentation

All students will give an oral presentation, related to an analysis of a dataset of their choosing (agreed upon by student and instructor).  Presentations should be 30-35 minutes in length, with an additional 5 minutes for group discussion.


A successful presentation should minimally address the following questions 1) why were the analyses performed done [why is it relevant?], 2) how were the data generated/analyzed, 3) what are the biological insights gained? 4) Discuss caveats and future directions.





Course Grading 


             Grading scale for graduates and undergraduates:

            90 – 100% = A

            80 – 89% = B

            70 – 79% = C

            60 – 69% = D

0        – 59% = F



Final Exam Information


              Will be replaced by a final presentation and written assignment





Mid-term Grade (for 100-400 level courses, and for undergraduates in 500 level courses)


Mid-term grades will be posted in myUK by the deadline established in the Academic Calendar (http://www.uky.edu/Registrar/AcademicCalendar.htm




Course Policies:


 



Submission of Assignments:



Presentations are expected to be presented orally. Written assignments may be emailed to the instructor (single spaced, 12 point font). Written assignments are due on or before 02/25/2022.



 



Attendance Policy. 



Students are expected to attend and participate in all classes.  See below for further detail.


Tentative Outline of Learning Process










1/21/22

Introduction to computational biology and the terminal (blast), file structure basics, data sources.



1/28/22

Introduction to machine commands and remote access installing and compiling programs, sequencing technologies.



2/4/22

Pre-proposals Group 1  …. Introduction to machine commands and remote access installing and compiling programs  Take Home Quiz.



2/11/22

Pre-proposals Group 2  ….Intro to assembling transcriptomes and simple genomes      



2/18/22

Assembling transcriptomes and simple genomes  Take Home Quiz



2/25/22

Transcriptional profiling and statistical analysis (from sequence data)  Due date for Written Assignment



3/4/22

Analysis of SNPs and genomic features     



3/11/22

Visualizing and interpreting genomic/ transcriptomic data Take Home Quiz



3/18/22

NO CLASS ACADEMIC HOLIDAY



3/25/22

Class Presentations



4/1/22

Class Presentations



4/8/22

Class Presentations



4/15/22

Class Presentations



4/22/22

Class Presentations



4/27/22

“UK Reading Days”









 

Midterm Grades for Undergraduate Students (Senate Rules 6.1.3.1)  

Midterm grades will be posted in myUK by the deadline established by the University Senate and published in the Academic Calendar.


Excused Absences (Senate Rules 5.2.5.2.1)

Senate Rules 5.2.5.2.1 defines the following as acceptable reasons for excused absences: 1. significant illness; 2. death of a family member; 3. trips for members of student organizations sponsored by an educational unit, trips for University classes, and trips for participation in intercollegiate athletic events; 4. major religious holidays; 5. interviews for graduate/professional school or full-time employment post-graduation; and 6. other circumstances found to fit “reasonable cause for nonattendance” by the instructor of record. Students should notify the professor of absences prior to class when possible.

 

If a course syllabus requires specific interactions (e.g., with the instructor or other students), in situations where a student’s total EXCUSED absences exceed 1/5 (or 20%) of the required interactions for the course, the student shall have the right to request and receive a "W," or the Instructor of Record may award an “I” for the course if the student declines a “W.” (Senate Rules 5.2.5.2.3.1)

 

If an attendance/interaction policy is not stated in the course syllabus or the policy does not include a penalty to the student, the Instructor cannot penalize the student for any unexcused absences. (Senate Rules 5.2.5.2.3.3)


Verification of Absences (Senate Rules 5.2.5.2.1)

Students may be asked to verify their absences in order for them to be considered excused. Senate Rule 5.2.5.2.1 states that faculty have the right to request appropriate verification when students claim an excused absence due to: significant illness; death in the household, trips for classes, trips sponsored by an educational unit and trips for participation related to intercollegiate athletic events; and interviews for full-time job opportunities after graduation and interviews for graduate and professional school. (Appropriate notification of absences due to University-related trips is required prior to the absence when feasible and in no case more than one week after the absence.)


Programs with learning activities mandated by accreditation or licensure agencies may establish, as a matter of policy, educational consequences for students who have so many excused absences that they cannot complete the mandated learning activities. Pursuant to Senate Rules 6.1.1, the published program policies and individual course syllabi must describe these consequences, which may include the student being moved to a different graduation cohort.


Religious Observances (Senate Rules 5.2.5.2.1(4))

Students anticipating an absence for a major religious holiday are responsible for notifying the instructor in writing of anticipated absences due to their observance of such holidays. Please check the course syllabus for the notification requirement. If no requirement is specified, two weeks prior to the absence is reasonable and should not be given any later. Information regarding major religious holidays may be obtained through the Ombud’s website or calling 859-257-3737.


Make-Up Work (Senate Rule 5.2.5.2.2)

Students missing any graded work due to an excused absence are responsible: for informing the Instructor of Record about their excused absence within one week following the period of the excused absence (except where prior notification is required); and for making up the missed work. The instructor must give the student an opportunity to make up the work and/or the exams missed due to the excused absence, and shall do so, if feasible, during the semester in which the absence occurred. The instructor shall provide the student with an opportunity to make up the graded work and may not simply calculate the student's grade on the basis of the other course requirements, unless the student agrees in writing.


For students who add a class after the first day of classes and miss graded work, the instructor shall provide the student with an opportunity to make up the graded work (quiz, exam, homework, etc.). The instructor may not simply calculate the student's grade on the basis of the other course requirements, unless the student agrees in writing.


Excused Absences and W/I, All Students (Senate Rule 5.2.5.2.3.1)

If a student has excused absences for more than one-fifth of the required interactions for a course, the student can request a "W." If the student declines a “W,” the Instructor of Record may award an “I” for the course.


Excused Absences Due to Military Duties (Senate Rule 5.2.5.2.3.2)

If a student must be absent for one-fifth or less of the required course interactions (e.g., class meetings) due to military duties, the following procedure apply:

  1. Once a student is aware of a call to duty, the student shall provide a copy of the military orders to the Director of the Veterans Resource Center. The student shall also provide the Director with a list of her/his courses and instructors.
  2. The Director will verify the orders with the appropriate military authority and on behalf of the military student, notify each Instructor of Record via Department Letterhead as to the known extent of the absence.
  3. The Instructor of Record shall not penalize the student’s absence in any way and shall provide accommodations and timeframes so that the student can make up missed assignments, quizzes, and tests in a mutually agreed upon manner.


Accommodations Due to Disability

If you have a documented disability that requires academic accommodations, please see me as soon as possible during scheduled office hours. In order to receive accommodations in this course, you must provide me with a Letter of Accommodation from the Disability Resource Center (DRC). The DRC coordinates campus disability services available to students with disabilities. Visit the DRC website, email the DRC, contact them by phone at (859) 257-2754, or visit their office on the corner of Rose Street and Huguelet Drive in the Multidisciplinary Science Building, Suite 407.


Non-Discrimination Statement and Title IX Information

UK is committed to providing a safe learning, living, and working environment for all members of the University community. The University maintains a comprehensive program which protects all members from discrimination, harassment, and sexual misconduct. For complete information about UK’s prohibition on discrimination and harassment on aspects such as race, color, ethnic origin, national origin, creed, religion, political belief, sex, and sexual orientation, please see the electronic version of UK’s Administrative Regulation 6:1 (“Policy on Discrimination and Harassment”) . In accordance with Title IX of the Education Amendments of 1972, the University prohibits discrimination and harassment on the basis of sex in academics, employment, and all of its programs and activities. Sexual misconduct is a form of sexual harassment in which one act is severe enough to create a hostile environment based on sex and is prohibited between members of the University community and shall not be tolerated. For more details, please see the electronic version of Administrative Regulations 6:2 (“Policy and Procedures for Addressing and Resolving Allegations of Sexual Assault, Stalking, Dating Violence, Domestic Violence, and Sexual Exploitation”). Complaints regarding violations of University policies on discrimination, harassment, and sexual misconduct are handled by the Office of Institutional Equity and Equal Opportunity (IEEO), which is located in 13 Main Building and can be reached by phone at (859) 257-8927. You can also visit the IEEO’s website


Faculty members are obligated to forward any report made by a student related to IEEO matters to the Office of Institutional Equity and Equal Opportunity. Students can confidentially report alleged incidences through the Violence Intervention and Prevention Center, Counseling Center, or University Health Services.


Academic Integrity– Prohibition on Plagiarism (Senate Rules 6.3.1)

Per University policy, students shall not plagiarize, cheat, or falsify or misuse academic records. Students are expected to adhere to University policy on cheating and plagiarism in all courses. The minimum penalty for a first offense is a zero on the assignment on which the offense occurred. If the offense is considered severe or the student has other academic offenses on their record, more serious penalties, up to suspension from the University may be imposed.


Plagiarism and cheating are serious breaches of academic conduct. Each student is advised to become familiar with the various forms of academic dishonesty as explained in the Code of Student Rights and Responsibilities. Complete information can be found on the Academic Ombud page. A plea of ignorance is not acceptable as a defense against the charge of academic dishonesty. It is important that you review this information as all ideas borrowed from others need to be properly credited.


Senate Rule 6.3.1 (see current Senate Rules) states that all academic work, written or otherwise, submitted by students to their instructors or other academic supervisors, is expected to be the result of their own thought, research, or self-expression. In cases where students feel unsure about a question of plagiarism involving their work, they are obliged to consult their instructors on the matter before submission.


When students submit work purporting to be their own, but which in any way borrows ideas, organization, wording, or content from another source without appropriate acknowledgment of the fact, the students are guilty of plagiarism.


Plagiarism includes reproducing someone else's work (including, but not limited to a published article, a book, a website, computer code, or a paper from a friend) without clear attribution. Plagiarism also includes the practice of employing or allowing another person to alter or revise the work which a student submits as his/her own, whoever that other person may be, except under specific circumstances (e.g. Writing Center review or peer review) allowed by the Instructor of Record or that person’s designee. Plagiarism may also include double submission, self-plagiarism, or unauthorized resubmission of one’s own work, as defined by the instructor.


Students may discuss assignments among themselves or with an instructor or tutor, except where prohibited by the Instructor of Record (e.g. individual take-home exams). However, the actual work must be done by the student, and the student alone, unless collaboration is allowed by the Instructor of Record (e.g. group projects).


When a student's assignment involves research in outside sources or information, the student must carefully acknowledge exactly what, where and how he/she has employed them. If the words of someone else are used, the student must put quotation marks around the passage in question and add an appropriate indication of its origin. Making simple changes while leaving the organization, content, and phraseology intact is plagiaristic. However, nothing in these Rules shall apply to those ideas, which are so generally and freely circulated as to be a part of the public domain.


Please note:  Any assignment you turn in may be submitted to an electronic database to check for plagiarism.


Academic Integrity – Prohibition on Cheating (Senate Rules 6.3.2)

Cheating is defined by its general usage. It includes, but is not limited to, the wrongfully giving, taking, or presenting any information or material by a student with the intent of aiding himself/herself or another on any academic work which is considered in any way in the determination of the final grade. The fact that a student could not have benefited from an action is not by itself proof that the action does not constitute cheating. Any question of definition shall be referred to the University Appeals Board.


Academic Integrity – Prohibition on Falsification/Misuse of Academic Records (SR 6.3.3)

Maintaining the integrity, accuracy, and appropriate privacy of student academic records is an essential administrative function of the University and a basic protection of all students. Accordingly, the actual or attempted falsification, theft, misrepresentation or other alteration or misuse of any official academic record of the University, specifically including knowingly having unauthorized access to such records or the unauthorized disclosure of information contained in such records, is a serious academic offense. As used in this context, "academic record" includes all paper and electronic versions of the partial or complete permanent academic record, all official and unofficial academic transcripts, application documents and admission credentials, and all academic record transaction documents. The minimum sanction for falsification, including the omission of information, or attempted falsification or other misuse of academic records as described in this section is suspension for one semester.


 

Mask and Social Distancing Policy

In accordance with University guidelines, students must wear UK-approved face coverings in the classroom and academic buildings (e.g., faculty offices, laboratories, libraries, performance/design studios, and common study areas where students might congregate). If UK-approved face coverings are not worn over the nose and mouth, students will be asked to leave the classroom.

 

Students should complete their daily online wellness screening before accessing university facilities and arriving to class.

 

Students should not move chairs or barriers in classrooms and should socially distance at all times, leaving a six (6) foot radius from other people. Masks and hand sanitizer can be found {specific location in building} if needed.

 

Students should leave enough space when entering and exiting a room. Students should not crowd doorways at the beginning or end of class.

 

Course Material Copyright Statement

The University of Kentucky Code of Student Conduct defines Invasion of Privacy as using electronic or other devices to make a photographic, audio, or video record of any person without their prior knowledge or consent when such a recording is likely to cause injury or distress.

 

Meetings of this course may be recorded. All video and audio recordings of lecturers and class meetings, provided by the instructors, are for educational use by students in this class only. They are available only through the Canvas shell for this course and are not to be copied, shared, or redistributed.

 

As addressed in the Code of Student Conduct, students are expected to follow appropriate university policies and maintain the security of linkblue accounts used to access recorded class materials. Recordings may not be reproduced, shared with those not enrolled in the class, or uploaded to other online environments.

 

If the instructor or a University of Kentucky office plans any other uses for the recordings, beyond this class, students identifiable in the recordings will be notified to request consent prior to such use. In anticipation of such cases, students may be asked to complete an “authorization of use” form by a faculty member.

 

Video and audio recordings by students are not permitted during the class unless the student has received prior permission from the instructor. Any sharing, distribution, and or uploading of these recordings outside of the parameters of the class is prohibited. Students with specific recording accommodations approved by the Disability Resource Center should present their official documentation to the instructor.

 

All content for this course, including handouts, assignments, and lectures are the intellectual property of the instructors and cannot be reproduced or sold without prior permission from the instructors. A student may use the material for reasonable educational and professional purposes extending beyond this class, such as studying for a comprehensive or qualifying examination in a degree program, preparing for a professional or certification examination, or to assist in fulfilling responsibilities at a job or internship.

 

Bias Incident Support Services

Bias Incident Support Services (BISS) provides confidential support and advocacy for any student, staff, or faculty member impacted by bias, hatred, and/or an act of identity-based violence.  BISS staff aid impacted parties in accessing campus and community resources, including the Bias Incident Response Team, the University’s official reporting system for acts that negatively impact a sense of belonging. Campus and community consultation and educational opportunities centered on inclusion, diversity, equity and belonging is a resource also provided by BISS. For more detailed information please visit the BISS website or contact them via email.

 

Counseling Center

The UK Counseling Center (UKCC) provides a range of confidential psychological services to students enrolled in 6 credit hours or more, psychoeducational outreach programming (including QPR suicide prevention), and consultation to members of the UK community (students, faculty, staff, administrators, parents, concerned others).  Please visit the website https://www.uky.edu/counselingcenter/  for more detailed information, or call 859.257.8701.

 

Martin Luther King Center

The Martin Luther King Center (MLKC) supports an inclusive learning environment where diversity and individual differences are understood, respected, and appreciated as a source of strength. The MLKC’s year-round programs and activities that focus on the importance of cultural awareness and cross-cultural understanding support its three primary goals: 1) sponsoring cultural and educational programming; 2) offering opportunities for student support and development; and 3) through programmatic linkages with a wide variety of civic and community agencies, promoting community outreach, engagement, and collaboration.  Students can reach the MLKC via phone at (859) 257-4130, by visiting them in Gatton Student Center Suite A230, via email, and by visiting the MLKC website.

 

Office of LGBTQ* Resources

UK is committed to supporting students and upholding the University’s efforts to promote inclusion among our community. UK faculty and staff employees support inclusion and diversity throughout the University, including the ways in which faculty structure classroom conversations and manage those dynamics. To assist in these efforts, students are welcome to provide the names and pronouns they prefer. One easy way to do this is by using the pronoun feature of UK’s Name Change Form. (More information about the form can be found on the Office of LGBTQ*’s website.) Otherwise, students can provide this information to faculty members directly.

 

Discrimination based on sexual orientation, gender expression, and gender identity is prohibited at UK. If you have questions about support, advocacy, and community-building services related to sexual orientation, gender expression, or gender identity, students are encouraged to visit the website of the Office of LGBTQ* Resources.

 

Veteran’s Resource Center

Being both a member of the military community and a student can bring some complexities. If you are a member of the military or a military veteran or dependent, please let me know when these challenges arise. Drill schedules, calls to active duty, mandatory training exercises, issues with GI Bill disbursement, etc. can complicate your academic life. Let me know if you experience complications and I will do my best to work with you.

 

The Veteran’s Resource Center (VRC) is a great resource for members of our military family. If you have questions regarding your VA benefits or other related issues, the VRC has a full complement of staff to assist you. The VRC also provides study and lounge space, as well as free printing. Please visit the VRC website, email the VRC, visit them in the basement of Erikson Hall, or call the director, Colonel Tony Dotson, at (859) 257-1148.

 

If you are a military student serving in the National Guard or Reserve, it is in your best interest to let all of your professors know that immediately. You might also consider sharing a copy of your training schedule. 

 

If you are a military student who is a member of the National Guard or Military Reserve and are called to duty for one-fifth or less of this semester, please help me help you! Once you become aware of the call to duty, provide a copy of your military orders to the Director of the Veterans Resource Center (contact information above). (Please also provide the Director with a list of all your current courses and instructors.) The Director will verify the orders with the appropriate military authority and on your behalf will notify me and your other instructors as to the known extent of the absence.  

I will not penalize your absence in any way and will work with you to create reasonable accommodations for making up missed assignments, quizzes, and tests.

 

Violence Intervention and Prevention (VIP) Center

If you experience an incident of sex- or gender-based discrimination or interpersonal violence, we encourage you to report it. While you may talk to a faculty member or TA/RA/GA, understand that as a "Responsible Employee" of the University these individuals MUST report any acts of violence (including verbal bullying and sexual harassment) to the University's Title IX Coordinator in the IEEO Office. If you would like to speak with someone who may be able to afford you confidentiality, you can visit the Violence Intervention and Prevention (VIP) Center’s  website (offices located in Frazee Hall, lower level; email them; or call (859) 257-3574), the Counseling Center’s (CC)  website  (106 Frazee Hall; (859) ), and the University Health Services (UHS) website; the VIP Center, CC, and UHS are confidential resources on campus. The VIP Center accepts walk-in appointments.

Center for Computational Sciences